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Proceedings Paper

Global analysis of distance distribution data for donor-acceptor pairs with different Forster distances
Author(s): Wieslaw M. Wiczk; Peggy S. Eis; Mayer N. Fishman; Michael L. Johnson; Joseph R. Lakowicz
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Paper Abstract

Distance distributions of a flexible molecule were recovered from steady-state fluorescence energy transfer measurements with the method suggested by Cantor and Pechukas In this method, the Forster distance (R0) is varied by attaching different donor-acceptor (D-A) pairs to the linker of interest. The distance distributions are recovered from the energy transfer efficiency measurements on D-A pairs with different R0s, followed by non-linear least squares fitting to distance distribution models. Thirteen compounds were synthesized with R0s ranging from 6-32 angstroms. Each compound contained a tryptamine donor linked by an alkyl chain (~10 carbon atoms) to one of thirteen different acceptors. The distance distributions were recovered by fitting the transfer efficiencies of the D-A pairs to both Gaussian and skewed Gaussian models. Our results indicate that the shape of the distribution is best described by a skewed Gaussian model. This is the first reported measurement of a skewed Gaussian distance distribution. In addition, the results of the Cantor and Pechuka's method and analysis were found to be consistent with distance distributions recovered from time-resolved frequency-domain fluorometry.

Paper Details

Date Published: 1 May 1990
PDF: 11 pages
Proc. SPIE 1204, Time-Resolved Laser Spectroscopy in Biochemistry II, (1 May 1990); doi: 10.1117/12.17757
Show Author Affiliations
Wieslaw M. Wiczk, Univ. of Maryland (United States)
Peggy S. Eis, Univ. of Maryland (United States)
Mayer N. Fishman, Univ. of Maryland (United States)
Michael L. Johnson, Univ. of Virginia (United States)
Joseph R. Lakowicz, Univ. of Maryland (United States)

Published in SPIE Proceedings Vol. 1204:
Time-Resolved Laser Spectroscopy in Biochemistry II
Joseph R. Lakowicz, Editor(s)

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