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Proceedings Paper

Diphenylhexatrienylpropanoylhydrazyl stachyose: a new fluorescence label for membrane research
Author(s): Josef Loidl; E. N. Ivessa; Edwin Kalb; Fritz Paltauf; Albin Hermetter
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Paper Abstract

Diphenylhexatrienylpropanoylhydrazyl stachyose (glyco-DPH), a new fluorescence probe, was synthesized. It inserts almost instantaneously into artificial phospholipid vesicles and biological membranes. Due to its large hydrophilic carbohydrate portion, it serves as an uncharged probe with a defined orientation within the membrane bilayer. Its usefulness to monitor lipid mobility by means of its fluorescence anisotropy could be demonstrated for dipalmitoylglycerophosphocholine at temperatures around the gel to liquid phase transition and the rigidifying effect of cholesterol on egg yolk phosphatidyicholine membranes. In addition, lipid mobility was determined for biological membrane systems such as yeast spheroplasts, yeast organelles, cultured human skin fibroblasts and compared with the 'fluidities’ of vesicles made of the corresponding lipid extracts. Bimodal Lorentzian lifetime distributions determined for glyco-DPH in vesicles of 1 -palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine showed that the label is distributed homogeneously within a phospholipid bilayer. Fluorescence microscopy of living (fibroblast) cells revealed selective labeling of the surface membrane with glyco-DPH under appropriate conditions.

Paper Details

Date Published: 1 May 1990
PDF: 5 pages
Proc. SPIE 1204, Time-Resolved Laser Spectroscopy in Biochemistry II, (1 May 1990); doi: 10.1117/12.17751
Show Author Affiliations
Josef Loidl, Graz Univ. of Technology (Austria)
E. N. Ivessa, Graz Univ. of Technology (Austria)
Edwin Kalb, Graz Univ. of Technology (Austria)
Fritz Paltauf, Graz Univ. of Technology (Austria)
Albin Hermetter, Graz Univ. of Technology (Austria)

Published in SPIE Proceedings Vol. 1204:
Time-Resolved Laser Spectroscopy in Biochemistry II
Joseph R. Lakowicz, Editor(s)

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