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Proceedings Paper

Time-resolved fluorescence anisotropy studies of mixed lipid bilayers
Author(s): A. S. Holmes; J. Cooper; David J. S. Birch; Robert E. Imhof
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Paper Abstract

Time-resolved fluorescence anisotropy of diphenylhexatriene (DPH) in various binary lipid mixtures of dimysterolphosphatidycholine (DMPC), dipalmitoylphosphatidylcholine (DPPC), distearoylphosphatidylcholine (DSPC) and dioleoylphosphatidylcholine (DOPC) has been studied. Evidence is given for phase separation in binary mixtures of DMPC/DSPC and DOPC/DSPC which is not found in DMPC/DPPC and DPPC/DSPC mixtures. A rising anisotropy decay has been observed for DPH in binary lipid mixtures containing DSPC and in DSPC liposomes at the phase transition which suggests a heterogeneous environment for the fluorophore at this temperature.

Paper Details

Date Published: 1 May 1990
PDF: 8 pages
Proc. SPIE 1204, Time-Resolved Laser Spectroscopy in Biochemistry II, (1 May 1990); doi: 10.1117/12.17745
Show Author Affiliations
A. S. Holmes, Univ. of Strathclyde (United Kingdom)
J. Cooper, ICI plc (United Kingdom)
David J. S. Birch, Univ. of Strathclyde (United Kingdom)
Robert E. Imhof, Univ. of Strathclyde (United Kingdom)

Published in SPIE Proceedings Vol. 1204:
Time-Resolved Laser Spectroscopy in Biochemistry II
Joseph R. Lakowicz, Editor(s)

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