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Proceedings Paper

Kinetics of tumor necrosis factor production by photodynamic-therapy-activated macrophages
Author(s): Harvey I. Pass; Steven Evans M.D.; Roger Perry M.D.; Wilbert Matthews
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Paper Abstract

The ability of photodynamic therapy (PDT) to activate macrophages and produce cytokines, specifically tumor necrosis factor (TNF), is unknown. Three day thioglycolate elicited macrophages were incubated with 25 ug/mi Photofrin II (P11) for 2 hour, after which they were subjected to 630 nm light with fluences of 0-1800 J/m. The amount of TNF produced in the system as well as macrophage viability was measured 1, 3, 6, and 18 hours after POT. The level of TNF produced by the macrophages was significantly elevated over control levels 6 hours after POT and the absolute level of tumor necrosis factor production was influenced by the treatment energy and the resulting macrophage cytotoxicity. These data suggest that POT therapy induced cytotoxicity in vivo may be amplified by macrophage stimulation to secrete cytokines and these cytokines may also participate in other direct/indirect photodynamic therapy effects, i.e. immunosuppression, vascular effects.

Paper Details

Date Published: 1 July 1990
PDF: 8 pages
Proc. SPIE 1203, Photodynamic Therapy: Mechanisms II, (1 July 1990); doi: 10.1117/12.17660
Show Author Affiliations
Harvey I. Pass, National Institutes of Health (United States)
Steven Evans M.D., National Institutes of Health (United States)
Roger Perry M.D., National Institutes of Health (United States)
Wilbert Matthews, National Institutes of Health (United States)

Published in SPIE Proceedings Vol. 1203:
Photodynamic Therapy: Mechanisms II
Thomas J. Dougherty, Editor(s)

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