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Proceedings Paper

Targetable N-(2-hydroxypropyl)methacrylamide copolymer-chlorin e6 conjugates
Author(s): Jindrich Kopecek; Nancy L. Krinick; B. Rihova; K. Ulbrich
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Paper Abstract

Two N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer-chlorin es-anti-Thy 1.2 antibody conjugates were synthesized. They differ in the method of antibody binding. in conjugate A, the polyclonal anti-Thy 1 .2 antibody was bound via NEamino groups of lysine residues. Conjugate B contained anti-Thy-I .2 antibodies bound via oxidized carbohydrate groups present near the hinge region of the F part of the antibody molecule. The photodynamic activities of these conjugates (and of appropriate controls) were evaluated on mouse splenocytes in vitro. Both conjugates (A and B) were more biologically active compared to the nontargetable conjugate (without antibody). All polymeric chiorin e6 conjugates were less toxic in the dark compared to free chlorin e6. Conjugate B was the most active; its activity at low concentrations was higher compared to free chlorin e6. The results demonstrate the importance of the chemistry of antibody binding on the biological activity of targetable polymeric drugs.

Paper Details

Date Published: 1 July 1990
PDF: 9 pages
Proc. SPIE 1203, Photodynamic Therapy: Mechanisms II, (1 July 1990); doi: 10.1117/12.17658
Show Author Affiliations
Jindrich Kopecek, Univ. of Utah (United States)
Nancy L. Krinick, Univ. of Utah (United States)
B. Rihova, Institute of Microbiology (Czech Republic)
K. Ulbrich, Institute of Macromolecular Chemistry (Czech Republic)

Published in SPIE Proceedings Vol. 1203:
Photodynamic Therapy: Mechanisms II
Thomas J. Dougherty, Editor(s)

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