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Proceedings Paper

Investigations concerning the determination of NADH concentrations using optical biopsy
Author(s): Juergen Beuthan; Thomas Bocher; Olaf Minet; Andre Roggan; Isabella Schmitt; A. Weber; Gerhard J. Mueller
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Paper Abstract

The intrinsic NADH autofluorescence intensity of biological tissue depends on the local, cellular concentration of this coenzyme. It plays a dominant role in the Krebs-Cycle and therefore serves as indicator for the vitality of the observed cells. Due to individually and locally varying boundary conditions and optical tissue properties, which are scattering coefficients, absorption coefficients and g-factors the fluorescence signal needs to be rescaled. One possible rescaling method is the theoretical derived Photon Migration Theory. Our new rescaling method is partly based on measurements and partly theoretical derived. By using the 4 information channels: LIF time-resolved signal, biochemical concentration measurements, Monte Carlo simulations with optical parameters and microscopic investigations we demonstrate that simultaneous detection of the fluorescence and the backscattering signal can easily and accurately provide rescaled, quantitative values for the NADH concentrations.

Paper Details

Date Published: 19 May 1994
PDF: 10 pages
Proc. SPIE 2135, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases, (19 May 1994); doi: 10.1117/12.176007
Show Author Affiliations
Juergen Beuthan, Freie Univ. Berlin (Germany)
Thomas Bocher, Freie Univ. Berlin (Germany)
Olaf Minet, Freie Univ. Berlin (Germany)
Andre Roggan, Freie Univ. Berlin (Germany)
Isabella Schmitt, Freie Univ. Berlin (Germany)
A. Weber, Freie Univ. Berlin (Germany)
Gerhard J. Mueller, Freie Univ. Berlin (Germany)

Published in SPIE Proceedings Vol. 2135:
Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases
Robert R. Alfano, Editor(s)

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