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Photodynamic treatment (PDT) of endometrium primary cultures serving as an in-vitro-model for endometriosis
Author(s): Wiebke Herter; Volker Viereck; J. Keckstein M.D.; Rudolf W. Steiner; Angelika C. Rueck
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Paper Abstract

As a new treatment model for endometriosis, photodynamic therapy (PDT) was applied to endometrium cultures. Endometriosis is a benign disease. Therefore primary cultures were used instead of cell lines. Endometrium is composed of epithelial and stromal cells which can also be found in primary culture. While stromal cells take a polygonal shape in culture, epithelial cells form cell colonies. PSIII (Photasan III), which is similar to hematorporphyrin derivate (HpD), meso-tetra (4-sulfonatophenyl) porphyrin (TPPS4), which posses a high fluorescence quantum yield and may be useful in fluorescence diagnosis of subtle endometriotic spots, and methylene blue (MB), a vital dye with phototoxic properties, were used as photosensitizers. Different sensitizer concentrations and incubation times were applied. The highest phototoxicity was observed for PSIII; TPPS4 and MB were less phototoxic. We compared our results with the sensitivity of cell lines described in the literature. The necessary irradiation to destroy stromal cells was relatively high but still in the same dimension as for cell lines. However they were even more sensitive than epithelial cells. This was true for all sensitizers used.

Paper Details

Date Published: 19 May 1994
PDF: 12 pages
Proc. SPIE 2135, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases, (19 May 1994); doi: 10.1117/12.176005
Show Author Affiliations
Wiebke Herter, Univ. Ulm (Germany)
Volker Viereck, Univ. Ulm (Germany)
J. Keckstein M.D., Univ. Ulm (Germany)
Rudolf W. Steiner, Institut fuer Lasertechnologien in der Medizin/Univ. Ulm (Germany)
Angelika C. Rueck, Institut fuer Lasertechnologien in der Medizin/Univ. Ulm (Germany)

Published in SPIE Proceedings Vol. 2135:
Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases
Robert R. Alfano, Editor(s)

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