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Proceedings Paper

Biochemical sensing in tissues: determination of fluorescent lifetimes in multiply scattering media using frequency-domain spectroscopy
Author(s): Christina L. Burch; Joseph R. Lakowicz; Eva Marie Sevick-Muraca
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Paper Abstract

Numerical solutions to the diffusion equation describing light propagation in tissues are presented. The results show that time- dependent measurements of light propagation can be used to monitor the fluorescent lifetimes of a fluorophore uniformly distrusted in tissues. With proper referencing, frequency-domain measurements of phase-shift, (Theta) , may allow quantitation of fluorescent lifetimes, (tau) , independent of changes in the local absorption and scattering properties. These results point to a new approach for noninvasive diagnostic monitoring through quantitation of fluorescent lifetimes, (tau) .

Paper Details

Date Published: 19 May 1994
PDF: 14 pages
Proc. SPIE 2135, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases, (19 May 1994); doi: 10.1117/12.176003
Show Author Affiliations
Christina L. Burch, Vanderbilt Univ. (United States)
Joseph R. Lakowicz, Univ. of Maryland/Baltimore School of Medicine (United States)
Eva Marie Sevick-Muraca, Vanderbilt Univ. (United States)

Published in SPIE Proceedings Vol. 2135:
Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases
Robert R. Alfano, Editor(s)

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