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Proceedings Paper

Monitoring of aqueous humor metabolites using Raman spectroscopy
Author(s): James P. Wicksted; Roel J. Erckens; Massoud Motamedi; Wayne F. March
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Paper Abstract

Laser Raman scattering has been used to monitor glucose and lactate metabolites within aqueous humor specimens obtained from nine human eyes during cataract surgery. Nine postmortem rabbit eyes were also investigated. Raman measurements were obtained using a single grating Raman spectrometer with a liquid nitrogen cooled CCD. A 514.5 nm line from an argon laser was used to illuminate capillaries containing several microliters of aqueous humor. A water background was subtracted from each of the aqueous humor Raman spectra. This experimental system was calibrated so that each metabolite in water could be measured down to 0.1 weight percent. Raman peaks indicative of the stretching vibrations of methylene and methyl groups associated with glucose and lactate, respectively, were observed in the human specimens. A second stretching mode characteristic of lactate between the carbon atom and either the carboxylic acid group or carboxylate ion group was also observed providing a distinguishing feature between the glucose and lactate Raman peaks. Similar structure was observed from the rabbit specimens, but these samples have recently been found to have been contaminated during euthanasia.

Paper Details

Date Published: 19 May 1994
PDF: 11 pages
Proc. SPIE 2135, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases, (19 May 1994); doi: 10.1117/12.176001
Show Author Affiliations
James P. Wicksted, Oklahoma State Univ. (United States)
Roel J. Erckens, Univ. of Texas Medical Branch/Galveston (United States)
Massoud Motamedi, Univ. of Texas Medical Branch/Galveston (United States)
Wayne F. March, Univ. of Texas Medical Branch/Galveston (United States)

Published in SPIE Proceedings Vol. 2135:
Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases
Robert R. Alfano, Editor(s)

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