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Proceedings Paper

New light sensor molecules of single-cell ciliates
Author(s): Nengbing Tao; Pill-Soon Song
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Paper Abstract

The unicellular ciliate, Stentor coeruleus, exhibits sensitive light-avoiding behavior. The photosensor stentorin showed a (M - H)- at 591.1304, which is in accord with the formula C34H23O10. Acetylated stentorin, when FAB-desorbed as (M + H)+, shows a series of ions indicating the presence of eight hydroxyl groups. Additional confirmation is a collisionally activated decomposition (CAD) spectrum of the (M + H)+ of the octaacetate. The NMR spectrum of stentorin shows characteristic signals of isopropyl groups. Similar studies indicate that photosensor blepharismin from Blepharisma japonicum is structurally different from stentorin. Time-resolved fluorescence decays indicated that a primary event occurs within a few picoseconds. The stimulus light signal absorbed/perceived by Stentor and possibly by Blepharisma, is apparently amplified by a transient calcium influx into the cell. Preliminary studies suggest that signal transduction in both organisms utilizes G-protein(s) as an initial transducer and a cGMP-phosphodiesterase as the effector system, analogous to the visual system of higher animals.

Paper Details

Date Published: 1 May 1994
PDF: 11 pages
Proc. SPIE 2189, Smart Structures and Materials 1994: Smart Materials, (1 May 1994); doi: 10.1117/12.174060
Show Author Affiliations
Nengbing Tao, Univ. of Nebraska/Lincoln (United States)
Pill-Soon Song, Univ. of Nebraska/Lincoln (United States)

Published in SPIE Proceedings Vol. 2189:
Smart Structures and Materials 1994: Smart Materials
Vijay K. Varadan, Editor(s)

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