Share Email Print

Proceedings Paper

High-resolution, multiple optical-mode confocal microscope: I. System design, image acquisition and 3D visualization
Author(s): Carol J. Cogswell; Kieran G. Larkin; John W. O'Byrne; Matthew R. Arnison
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

We have designed and constructed an experimental confocal specimen-scanning microscope which has the capability of producing high resolution 3D images in a variety of optical modes, many of which are not currently available on commercial confocal microscopes. The transmission Nomarski differential interference contrast mode is particularly interesting because it can be utilized to image small changes in refractive index within complex biological specimens which are transparent in standard brightfield. The three-color reflection configuration can produce a color 3D image, which means that stained or pigmented objects will be similar in appearance to images obtained from conventional white light microscopes which makes them more recognizable.

Paper Details

Date Published: 4 April 1994
PDF: 7 pages
Proc. SPIE 2184, Three-Dimensional Microscopy: Image Acquisition and Processing, (4 April 1994); doi: 10.1117/12.172105
Show Author Affiliations
Carol J. Cogswell, Univ. of Sydney (Australia)
Kieran G. Larkin, Univ. of Sydney (Australia)
John W. O'Byrne, Univ. of Sydney (Australia)
Matthew R. Arnison, Univ. of Sydney (Australia)

Published in SPIE Proceedings Vol. 2184:
Three-Dimensional Microscopy: Image Acquisition and Processing
Carol J. Cogswell; Kjell Carlsson, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?