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Proceedings Paper

Application of digital image quality criteria to optimize the confocal microscope setup
Author(s): Andres Kriete
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Paper Abstract

This paper discusses how digital image quality criteria help to optimize image quality, in particular for applications in laser scanning microscopy. Image quality considerations offer a uniform description of the available transfer characteristics, which are summed up and weighted properly to finally represent the system by a single number. In the spatial domain we can measure sharpness and contrast of the (digital) volumes by analyzing intensities and their local dependencies in a statistical fashion. This includes sum modulus difference, gray level variance, and lateral inhibition. Based on information theory, the criterion volume fidelity takes into account the knowledge of the spatial structure of a test object and compares the intensities with those present in the final digital image. Applications presented here include measurement of image quality improvement when going from non-confocal to confocal imaging, testing of new confocal system designs and the evaluation of digital post-processing methods. Limitations in the presence of noise are discussed.

Paper Details

Date Published: 4 April 1994
PDF: 11 pages
Proc. SPIE 2184, Three-Dimensional Microscopy: Image Acquisition and Processing, (4 April 1994); doi: 10.1117/12.172094
Show Author Affiliations
Andres Kriete, Univ. Giessen (Germany)

Published in SPIE Proceedings Vol. 2184:
Three-Dimensional Microscopy: Image Acquisition and Processing
Carol J. Cogswell; Kjell Carlsson, Editor(s)

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