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Proceedings Paper

Comparative analysis of toxin detection in biological and enviromental samples
Author(s): Robert A. Ogert; James Burans; Tom O'Brien; Frances S. Ligler
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Paper Abstract

The basic recognition schemes underlying the principles of standard enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) protocols are increasingly being adapted for use with new detection devices. A direct comparison was made using a fiber optic biosensor that employs evanescent wave detection and an ELISA using avidin-biotin. The assays were developed for the detection of Ricinus communis agglutinin II, also known as ricin or RCA60. Detection limits between the two methods were comparable for ricin in phosphate buffered saline (PBS), however results in complex samples differed slightly. In PBS, sensitivity for ricin was 1 ng/ml using the fiber optic device and 500 pg/ml using the ELISA. The fiber optic sensor could not detect ricin directly in urine or serum spiked with 5 ng/ml ricin, however, the ELISA showed detection but at reduced levels to the PBS control.

Paper Details

Date Published: 10 March 1994
PDF: 8 pages
Proc. SPIE 2068, Chemical, Biochemical, and Environmental Fiber Sensors V, (10 March 1994); doi: 10.1117/12.170677
Show Author Affiliations
Robert A. Ogert, Naval Research Lab (United States)
James Burans, Naval Medical Research Institute (United States)
Tom O'Brien, Naval Medical Research Institute (United States)
Frances S. Ligler, Naval Research Lab. (United States)

Published in SPIE Proceedings Vol. 2068:
Chemical, Biochemical, and Environmental Fiber Sensors V
Robert A. Lieberman, Editor(s)

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