Recently, we have described a fiber optic biosensor specific for zinc that transduces the presence of the metal as a shift in the emission of a fluorescent sulfonamide inhibitor that binds to a metalloenzyme, human erythrocyte carbonic anhydrase, if and only if the metal is present. Due to fiber photoluminescence and calibration issues, we decided to determine if comparable results could be obtained by sensing based on fluorescence lifetime changes, as recently described by Demas, Wolfbeis, Lakowicz, and others. Results of these experiments are included, as well as a discussion of the dynamic range of the method.

Date Published: 10 March 1994
PDF: 11 pages
Proc. SPIE 2068, Chemical, Biochemical, and Environmental Fiber Sensors V, (10 March 1994); doi: 10.1117/12.170676

Published in SPIE Proceedings Vol. 2068:
Chemical, Biochemical, and Environmental Fiber Sensors V
Robert A. Lieberman, Editor(s)