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Proceedings Paper

Subcellular storage compartments of bacteriopheophorbide sensitizers
Author(s): Joerg G. Moser; U. Dembeck; M. Hubert; Bernhard Spengler; Rainer Bayer; Birgit Wagner
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Paper Abstract

Fluorescence colocalization with the Golgi specific stain, NBD-ceramide, and the mitochondrial localizing stain, Rhodamine 123, confirmed the earlier assumption that the Golgi apparatus is one of the prominent storage compartments for bacteriopheophorbide esters in OAT 75 SCLC cells and several amelanotic melanoma cell lines (A375, Melur SP18, SkAMel 25). Furthermore, a diffuse staining of mitochondria, of non-structured cytoplasm, and an additional storage in melanine vesicles of the amelanotic melanoma cells suggests further storage compartments with quantitatively different contributions to the phototoxicity of bacteriochlorophyll-derived photosensitizers. Independent observations of early phototoxic effects on microfilamentous networks, enzymatic activities (succinate dehydrogenase, lactate dehydrogenase), and redistribution phenomena following primary uptake of the sensitizers let us assume that only a part of the 108 molecules taken up by a cell contribute directly to phototoxicity. Thus it may be asked if a proper subcellular positioning of only a few sensitizer molecules may have similar phototoxic effects as the huge amounts stored at apparently ineffective sites.

Paper Details

Date Published: 1 March 1994
PDF: 7 pages
Proc. SPIE 2078, Photodynamic Therapy of Cancer, (1 March 1994); doi: 10.1117/12.168704
Show Author Affiliations
Joerg G. Moser, Heinrich-Heine-Univ. (Germany)
U. Dembeck, Heinrich-Heine-Univ. (Germany)
M. Hubert, Heinrich-Heine-Univ. (Germany)
Bernhard Spengler, Heinrich-Heine-Univ. (Germany)
Rainer Bayer, Heinrich-Heine-Univ. (Germany)
Birgit Wagner, Heinrich-Heine-Univ. (Germany)

Published in SPIE Proceedings Vol. 2078:
Photodynamic Therapy of Cancer
Giulio Jori; Johan Moan; Willem M. Star, Editor(s)

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