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Proceedings Paper

Kinetics of the photodynamic damage of phthalocyanine-loaded cultured K562 cells studied by means of flow cytometry
Author(s): Elena B. Chernyaeva; Jan Greve; Bart G. de Grooth
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Paper Abstract

The kinetics of cellular damage after photodynamic action was studied by means of flow- cytometry. Cultured K562 cells were loaded with tetrasulphonated aluminum phthalocyanine and propidium iodide, acridine orange and rhodamine 123 were used to assess the damage done to the cells. Exposure of phthalocyanine-loaded cells to light resulted in the following order of events: increase of the intracellular Pc fluorescence -> mitochondrial damage -> changes of the cell surface and/or volume -> plasma membrane potential vanishing -> severe damage of cell plasma membrane. For all these processes it takes approximately two hours to reach the stationary state.

Paper Details

Date Published: 1 March 1994
PDF: 12 pages
Proc. SPIE 2078, Photodynamic Therapy of Cancer, (1 March 1994); doi: 10.1117/12.168681
Show Author Affiliations
Elena B. Chernyaeva, Moscow State Univ. (Russia)
Jan Greve, Univ. of Twente (Netherlands)
Bart G. de Grooth, Univ. of Twente (Netherlands)

Published in SPIE Proceedings Vol. 2078:
Photodynamic Therapy of Cancer
Giulio Jori; Johan Moan; Willem M. Star, Editor(s)

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