Proceedings Paper
High-resolution microscopic techniques for probing subcellular changes during photodynamic therapy| Format | Member Price | Non-Member Price |
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Paper Abstract
Subcellular changes during photodynamic therapy (PDT) were probed simultaneously with two different microscopic techniques. These techniques include differential interference contrast (DIC) microscopy and fluorescence microscopy, in combination with video-contrast enhancement, averaging techniques, and digital image processing. The different cell organelles were identified by specific markers, such as rhodamine 123 and acridine orange. Subcellular morphological changes in fibroblastoid cells (3T3) and epithelial cells (RR 1022), incubated with the photosensitizer meso-tetra(4-sulfonatophenyl)porphyrin (TPPS4), were investigated during PDT treatment and correlated with the fluorescence pattern.
Paper Details
Date Published: 1 February 1994
PDF: 7 pages
Proc. SPIE 2083, Microscopy, Holography, and Interferometry in Biomedicine, (1 February 1994); doi: 10.1117/12.167423
Published in SPIE Proceedings Vol. 2083:
Microscopy, Holography, and Interferometry in Biomedicine
Adolf Friedrich Fercher; Aaron Lewis; Halina Podbielska M.D.; Herbert Schneckenburger; Tony Wilson, Editor(s)
PDF: 7 pages
Proc. SPIE 2083, Microscopy, Holography, and Interferometry in Biomedicine, (1 February 1994); doi: 10.1117/12.167423
Show Author Affiliations
Michael H. Gschwend, Univ. Ulm (Germany)
Herbert Schneckenburger, Univ. Ulm (Germany)
Wolfgang S. L. Strauss, Univ. Ulm (Germany)
Herbert Schneckenburger, Univ. Ulm (Germany)
Wolfgang S. L. Strauss, Univ. Ulm (Germany)
Published in SPIE Proceedings Vol. 2083:
Microscopy, Holography, and Interferometry in Biomedicine
Adolf Friedrich Fercher; Aaron Lewis; Halina Podbielska M.D.; Herbert Schneckenburger; Tony Wilson, Editor(s)
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