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Proceedings Paper

Liquid chromatography combined with visible diode-laser-induced fluorescence detection for photosensitizers
Author(s): Arjan J.G. Mank; Cees Gooijer; Henk Lingeman; Nel H. Velthorst; Udo A. T. Brinkman
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Paper Abstract

A combination of conventional-size liquid chromatography and visible diode-laser induced fluorescence (DIO-LIF) detection has been developed and applied for the detection of a model photosensitizer, AlPcS2, in biological samples. Conventional fluorimetry was found to give unsatisfactory information. A detection limit of 2.1 X 10-12 M (S/N equals 3) was obtained for AlPcS2 in both rat urine and faeces extracts using the DIO-LIF system, 20-fold lower than obtained with a conventional fluorescence detection system, when used in combination with chromatography.

Paper Details

Date Published: 1 February 1994
PDF: 12 pages
Proc. SPIE 2084, Biomedical Optoelectronic Devices and Systems, (1 February 1994); doi: 10.1117/12.167312
Show Author Affiliations
Arjan J.G. Mank, Free Univ. Amsterdam (Netherlands)
Cees Gooijer, Free Univ. Amsterdam (Netherlands)
Henk Lingeman, Free Univ. Amsterdam (Netherlands)
Nel H. Velthorst, Free Univ. Amsterdam (Netherlands)
Udo A. T. Brinkman, Free Univ. Amsterdam (Netherlands)

Published in SPIE Proceedings Vol. 2084:
Biomedical Optoelectronic Devices and Systems
Nathan I. Croitoru; Riccardo Pratesi, Editor(s)

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