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Proceedings Paper

Tissue diagnosis using autofluorescence
Author(s): Wolfgang Lohmann; Wolf-Bernhard Schill; Dieter Bucher; Theofried Peters; M. Nilles; Andreas Schulz; Rainer M. Bohle; Werner Schramm
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Paper Abstract

The fluorescence response of biological tissue (excited at 366 nm or 337 nm) depends on its condition: the intensity is rather low in cancer tissue and slightly higher at the edge of the tumor than in healthy tissue. This technique is non-invasive, non-destructive and is based on the native fluorophore NADH. Fluorescence images obtained with unstained, unfixed cryosections match the histological images. Since the cryosections can be prepared and evaluated in less than 5 min, this technique can also be used as a fast cut technique to determine e.g. the diagnosis of a biopsy sample. Additional investigations have shown that the fluorophore seems to be attached to elastic fibers.

Paper Details

Date Published: 15 January 1994
PDF: 15 pages
Proc. SPIE 2081, Optical Biopsy, (15 January 1994); doi: 10.1117/12.166825
Show Author Affiliations
Wolfgang Lohmann, Univ. of Giessen (Germany)
Wolf-Bernhard Schill, Univ. of Giessen (Germany)
Dieter Bucher, Univ. of Giessen (Germany)
Theofried Peters, Univ. of Giessen (Germany)
M. Nilles, Univ. of Giessen (Germany)
Andreas Schulz, Univ. of Giessen (Germany)
Rainer M. Bohle, Univ. of Giessen (Germany)
Werner Schramm, Research Institute for Molecular Pharmacology (Germany)

Published in SPIE Proceedings Vol. 2081:
Optical Biopsy
Rinaldo Cubeddu; Sune Svanberg; Hubert van den Bergh, Editor(s)

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