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Proceedings Paper

Use of particle beam LC/FTIR spectrometry for the structural analysis of globular proteins: preliminary studies with beta-lactoglobulin
Author(s): Vincent E. Turula; James A. de Haseth
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Paper Abstract

The Particle Beam apparatus can be used to desolvate proteins in preparation of IR measurements. Several experiments have been designed to determine whether the operation of the particle beam apparatus causes alternation to the complex structural features of globular proteins, and whether it produces a solid-state spectrum representative of the aqueous solution structure. (beta) -Lactoglobulin ((beta) LG), a milk protein with a structure well- characterized by infrared spectrometry, was chosen for this study. It is shown here that the structural integrity of this protein is maintained when nebulized, desolvated from solution, and deposited onto the IR-substrate. It is also possible to induce structural changes in proteins with the particle beam and a carrier-liquid concentration gradient.

Paper Details

Date Published: 31 January 1994
PDF: 2 pages
Proc. SPIE 2089, 9th International Conference on Fourier Transform Spectroscopy, (31 January 1994); doi: 10.1117/12.166707
Show Author Affiliations
Vincent E. Turula, Univ. of Georgia (United States)
James A. de Haseth, Univ. of Georgia (United States)

Published in SPIE Proceedings Vol. 2089:
9th International Conference on Fourier Transform Spectroscopy
John E. Bertie; Hal Wieser, Editor(s)

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