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Proceedings Paper

Solvent and metal ion effects on the conformation of cyclosporin
Author(s): R. A. Shaw; Henry H. Mantsch; Babur Z. Chowdhry
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Paper Abstract

Infrared spectra of cyclosporin A (CsA) and three analogues CsC, CsD, and CsH have been measured (1) in a variety of organic solvents, and (2) in acetonitrile in the presence of lithium, sodium, magnesium, and calcium ions. The amide I (CequalsO stretching) absorption pattern shows a systematic trend with increasing solvent polarity. The spectral changes indicate that polar solvents disrupt two of the four intramolecular hydrogen bonds, leaving the first two hydrogen bonds of the (beta) -sheet structure intact. Interaction of CsA, CsC, or CsD with the monovalent cations Li+ and Na+ in acetonitrile yields spectra that are virtually identical to one another. The spectra suggest that several carbonyl groups bind simultaneously to the metal ion. In contrast the spectra suggest strong binding by Ca2+ and Mg2+ to one or two specific CequalsO groups, as evidenced by very low frequency CequalsO stretching bands observed at ca. 1600 cm-1.

Paper Details

Date Published: 31 January 1994
PDF: 2 pages
Proc. SPIE 2089, 9th International Conference on Fourier Transform Spectroscopy, (31 January 1994); doi: 10.1117/12.166699
Show Author Affiliations
R. A. Shaw, National Research Council Canada (Canada)
Henry H. Mantsch, National Research Council Canada (Canada)
Babur Z. Chowdhry, Univ. of Greenwich (United Kingdom)

Published in SPIE Proceedings Vol. 2089:
9th International Conference on Fourier Transform Spectroscopy
John E. Bertie; Hal Wieser, Editor(s)

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