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Proceedings Paper

Combined IR and 31P NMR method for determining fractional composition in binary phospholipid monolayer films. Applications to phosphocholine: Phosphoglycerol mixtures
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Paper Abstract

A method has been developed to determine the exact fractional composition in binary mixtures of phospholipids at the air-water (A/W) interface by ATR-IR spectroscopy in combination with 31P NMR spectroscopy and Langmuir-Blodgett surface chemistry. The procedure utilizes the wavenumber shift observed upon the synthetic replacement of hydrogen with deuterium in the lipid acyl chains to separate the vibrational bands due to each component in mixtures of deuterated and normally protiated lipids. The C-H and C-D integrated areas obtained from the IR spectra of the binary mixture are correlated with the exact mole fractions as determined using high resolution 31P NMR spectroscopy. We have used this technique to measure the fractional composition of binary mixtures of phospholipids at the A/W interface containing acyl chain perdeutereated phosphocholine lipids in combination with several protiated phosphoglycerol lipids.

Paper Details

Date Published: 31 January 1994
PDF: 2 pages
Proc. SPIE 2089, 9th International Conference on Fourier Transform Spectroscopy, (31 January 1994); doi: 10.1117/12.166697
Show Author Affiliations
Amy D. Williams, Univ. of Georgia (United States)
Richard A. Dluhy, Univ. of Georgia (United States)

Published in SPIE Proceedings Vol. 2089:
9th International Conference on Fourier Transform Spectroscopy
John E. Bertie; Hal Wieser, Editor(s)

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