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Proceedings Paper

Crystallographic phase retrieval through image processing under constraints
Author(s): Kam Y. J. Zhang
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Paper Abstract

The crystallographic image processing techniques of Sayre's equation, molecular averaging, solvent flattening and histogram matching are combined in an integrated procedure for macromolecular phase retrieval. It employs the constraints of the local shape of electron density, equal molecules, solvent flatness and correct electron density distribution. These constraints on electron density image are satisfied simultaneously by solving a system of non- linear equations using fast Fourier transform. The electron density image is further filtered under the constraint of observed diffraction amplitudes. The effect of each constraint on phase retrieval is examined. The constraints are found to work synergistically in phase retrieval. Test results on 2Zn insulin are presented.

Paper Details

Date Published: 9 November 1993
PDF: 9 pages
Proc. SPIE 2029, Digital Image Recovery and Synthesis II, (9 November 1993); doi: 10.1117/12.161989
Show Author Affiliations
Kam Y. J. Zhang, Univ. of California/Los Angeles (United States)

Published in SPIE Proceedings Vol. 2029:
Digital Image Recovery and Synthesis II
Paul S. Idell, Editor(s)

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