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Proceedings Paper

Picosecond 3D fluorescence and absorption spectroscopy of a manipulated microparticle
Author(s): Keiji Sasaki
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Paper Abstract

Dynamic microspectroscopy systems have been developed for elucidating photophysical and photochemical processes occuring in small volumes. The first system concerns fluorescence spectroscopic measurements based on a timecorrelated single photon counting and a confocal fluorescence microscope, which makes it possible to observe the fluorescence dynamics with both picosecond time-resolution and micrometer three-dimensional space-resolution. The second system is for absorption spectroscopic measurements. Picosecond pump and probe beams are coaxially and confocally introduced into a microscope, giving the depth profile in addition to lateral information. These systems are further combined with a trapping laser which optically tweezers a single microparticle against its Brownian motion. Laser trapping thus introduces the precise time- and space-resolved measurement of particles. To analyze rise and decay curves as a function of position and wavelength, a new fast and accurate analyzing method using a convolved autoregressive model has been proposed. The computation time of this method is much shorter than that of the conventional methods. The present systems have been applied to various kinds of microparticles such as microcapsules, polymer latexes, and oil droplets.

Paper Details

Date Published: 24 September 1993
PDF: 8 pages
Proc. SPIE 1711, High-Performance Optical Spectrometry, (24 September 1993); doi: 10.1117/12.155641
Show Author Affiliations
Keiji Sasaki, Research Development Corp. of Japan (Japan)

Published in SPIE Proceedings Vol. 1711:
High-Performance Optical Spectrometry
Maksymilian Pluta; Aleksandra Kopystynska; Mariusz Szyjer, Editor(s)

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