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Proceedings Paper

Polarized light scattering as a means of detecting heat and UV-induced changes in bacteria and bacterial spores
Author(s): Willem Pieter Van De Merwe; Burt V. Bronk
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Paper Abstract

In the present paper we report on initial attempts to correlate changes in polarized light scattering signals from bacteria and bacterial spores with changes in physical parameters as a result of heat and UV exposure. Clear reproducible changes can be observed, but quantitative correlation such as measuring the degree of germination or survivability needs further study. Average cell size changes can be measured quickly in bacteria such as after UV exposure or during the growth cycle, making the used technique very valuable to monitor changes. Computer modelling is needed to distinguish effects caused by size changes and by changes in index of refraction.

Paper Details

Date Published: 21 July 1993
PDF: 6 pages
Proc. SPIE 1884, Static and Dynamic Light Scattering in Medicine and Biology, (21 July 1993);
Show Author Affiliations
Willem Pieter Van De Merwe, Uniformed Services Univ. of the Health Sciences (United States)
Burt V. Bronk, U.S. Army ERDEC (United States)

Published in SPIE Proceedings Vol. 1884:
Static and Dynamic Light Scattering in Medicine and Biology
Ralph J. Nossal; Robert Pecora; Alexander V. Priezzhev, Editor(s)

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