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Proceedings Paper

New advances in 3D visualization of the cornea
Author(s): Barry R. Masters
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Paper Abstract

This paper demonstrates the three-dimensional visualization of the ex vivo rabbit cornea. The cornea is an ideal test specimen to evaluate techniques for 3-D reconstruction and visualization of unstained, unfixed, transparent living tissues from a stack of optical sections. The 0.4 mm thick, transparent, specimen has been optically sectioned into 365 sections using a laser scanning confocal microscope with a water immersion objective. Isometric sampling resulted in near-cubic voxels which compensated for the reduced microscopic resolution in the z axis as compared to x and y resolution. This paper demonstrates the first three-dimensional visualization of the posterior stroma and the basal epithelium of the ex vivo rabbit cornea.

Paper Details

Date Published: 24 June 1993
PDF: 14 pages
Proc. SPIE 1877, Ophthalmic Technologies III, (24 June 1993); doi: 10.1117/12.147516
Show Author Affiliations
Barry R. Masters, Uniformed Services Univ. of the Health Sciences (United States)

Published in SPIE Proceedings Vol. 1877:
Ophthalmic Technologies III
Jean-Marie A. Parel; Qiushi Ren, Editor(s)

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