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Proceedings Paper

Laser-assisted cell fusion and cytoplast transfer in early mammalian embryos
Author(s): Annette Clement-Sengewald; Karin Schutze; A. Heinze; G. A. Palma; H. Poesl; G. Brem
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Paper Abstract

A UV-laser microbeam was successfully used to induce fusion of early embryonic cells. The developmental capacity of the laser-fused cells was examined using in vitro culture methods. Blastomeres within mouse two-cell embryos were fused with 3 - 10 subsequent laser pulses in order to produce tetraploid embryos. Thirty-one percent of the laser treated embryos fused and 10% of those developed to the morula or blastocyst stage. With 1 - 10 successive laser pulses cattle oocytes were fused with cytoplasts. Thirty-six percent of the laser treated cells fused and 10% of those cleaved to the 6- and 8-cell stage. These preliminary results indicate that a UV- laser microbeam combined with an optical tweezers may facilitate the manipulation of embryonic cells and can be a helpful tool in polyploidy studies and in cytoplasmic transfer experiments.

Paper Details

Date Published: 1 July 1993
PDF: 8 pages
Proc. SPIE 1876, Lasers in Otolaryngology, Dermatology, and Tissue Welding, (1 July 1993); doi: 10.1117/12.147030
Show Author Affiliations
Annette Clement-Sengewald, Bayerische Klonierungsforschungs-GmbH & Co. KG (Germany)
Karin Schutze, Applikatives Laserzentrum Harlaching (Germany)
A. Heinze, Applikatives Laserzentrum Harlaching (Germany)
G. A. Palma, Bayerische Klonierungsforschungs-GmbH & Co. KG (Germany)
H. Poesl, Applikatives Laserzentrum Harlaching (Germany)
G. Brem, Ludwig-Maximilians-Univ. Muenchen and Bayerische Klonierungsforschungs-GmbH & Co. KG (Germany)

Published in SPIE Proceedings Vol. 1876:
Lasers in Otolaryngology, Dermatology, and Tissue Welding
R. Rox Anderson M.D.; Lawrence S. Bass M.D.; Stanley M. Shapshay M.D.; John V. White M.D.; Rodney A. White, Editor(s)

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