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Proceedings Paper

Ultrasensitive detection of single molecules in flowing sample streams by laser-induced fluorescence
Author(s): Peter M. Goodwin; Charles W. Wilkerson Jr.; W. Patrick Ambrose; Richard A. Keller
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Paper Abstract

We report here on the detection and fluorescence lifetime measurement of single Rhodamine- 110 molecules in a flowing, aqueous sample stream. Time-correlated photon counting (TCPC) used in combination with pulsed excitation allows for the detection, in the presence of significant prompt Raman and Rayleigh background, of photon bursts due to single fluorescent molecules passing through a small detection volume (approximately 1 pL). The fluorescence lifetime of a detected molecule is estimated from the decay curve complied from photon arrival times in the burst.

Paper Details

Date Published: 24 June 1993
PDF: 11 pages
Proc. SPIE 1895, Ultrasensitive Laboratory Diagnostics, (24 June 1993); doi: 10.1117/12.146731
Show Author Affiliations
Peter M. Goodwin, Los Alamos National Lab. (United States)
Charles W. Wilkerson Jr., Los Alamos National Lab. (United States)
W. Patrick Ambrose, Los Alamos National Lab. (United States)
Richard A. Keller, Los Alamos National Lab. (United States)

Published in SPIE Proceedings Vol. 1895:
Ultrasensitive Laboratory Diagnostics
Gerald E. Cohn, Editor(s)

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