Chemiluminescence (CL) detection offers potential for high sensitivity immunoassays (CLIAs). Several approaches were attempted to automate CL measurements. Those include the use of photographic film, clear microtitration plates, and magnetic separation. We describe a photon counting detection apparatus that performs (CLIA) measurements. The CL detector moves toward a disposable reaction vessel to create a light-tight seal and then triggers and integrates a CL signal. The capture uses antibody coated polystyrene microparticles. A porous matrix, which is a part of a disposable reaction tray, entraps the microparticle-captured reaction product. The CL signal emanated off the immune complex immobilized by the porous matrix is detected. The detection system is a part of a fully automated immunoassay analyzer. Methods of achieving high sensitivities are discussed.

Date Published: 24 June 1993
PDF: 13 pages
Proc. SPIE 1895, Ultrasensitive Laboratory Diagnostics, (24 June 1993); doi: 10.1117/12.146728

Published in SPIE Proceedings Vol. 1895:
Ultrasensitive Laboratory Diagnostics
Gerald E. Cohn, Editor(s)