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Proceedings Paper

Investigations in high-speed DNA sequencing
Author(s): Rainer Erdmann; Claus A. M. Seidel; F. Schubert; Edgar O. Klose
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Paper Abstract

The application of a rapid time correlated single photon counting (TCSPC) apparatus on the investigation of fluorescent labelled DNA is presented. It could be shown that the investigated dye coumarin 120 can be used as a marker for a one dye-one lane electrophoresis-concept in DNA sequencing. This is possible because of the interaction between the dye and the four different DNA bases, which is based on a photoinduced electron transfer. A distinguishing of the bases is possible because of the different decay times. A fluorescein derivative also showed excellent fluorescence after labelling. It can be used as a marker for common electrophoresis- concepts, but the interaction was too weak for a secure base distinguishing a one dye-one lane. Now further investigations are under way to find new labeling positions resulting in stronger interactions. Additionally, we are looking for dyes in the red spectral range to improve the sensitivity of detection.

Paper Details

Date Published: 24 June 1993
PDF: 7 pages
Proc. SPIE 1895, Ultrasensitive Laboratory Diagnostics, (24 June 1993); doi: 10.1117/12.146726
Show Author Affiliations
Rainer Erdmann, GNF e.V. (Germany)
Claus A. M. Seidel, Ruprecht-Karls-Univ. Heidelberg (Germany)
F. Schubert, Humboldt Univ. Berlin (Germany)
Edgar O. Klose, GNF e.V. (Germany)

Published in SPIE Proceedings Vol. 1895:
Ultrasensitive Laboratory Diagnostics
Gerald E. Cohn, Editor(s)

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