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Proceedings Paper

DNA sequencing by single molecule detection of labeled nucleotides sequentially cleaved from a single strand of DNA
Author(s): Peter M. Goodwin; Jay A. Schecker; Charles W. Wilkerson Jr.; Mark L. Hammond; W. Patrick Ambrose; James H. Jett; John C. Martin; Babetta L. Marrone; Richard A. Keller; Alberto Haces; Po-Jen Shih; John D. Harding
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Paper Abstract

We are developing a laser-based technique for the rapid sequencing of large DNA fragments (several kb in size) at a rate of 100 to 1000 bases per second. Our approach relies on fluorescent labeling of the bases in a single fragment of DNA, attachment of this labeled DNA fragment to a support, movement of the supported DNA into a flowing sample stream, sequential cleavage of the end nucleotide from the DNA fragment with an exonuclease, and detection of the individual fluorescently labeled bases by laser-induced fluorescence.

Paper Details

Date Published: 24 June 1993
PDF: 5 pages
Proc. SPIE 1891, Advances in DNA Sequencing Technology, (24 June 1993); doi: 10.1117/12.146712
Show Author Affiliations
Peter M. Goodwin, Los Alamos National Lab. (United States)
Jay A. Schecker, Los Alamos National Lab. (United States)
Charles W. Wilkerson Jr., Los Alamos National Lab. (United States)
Mark L. Hammond, Los Alamos National Lab. (United States)
W. Patrick Ambrose, Los Alamos National Lab. (United States)
James H. Jett, Los Alamos National Lab. (United States)
John C. Martin, Los Alamos National Lab. (United States)
Babetta L. Marrone, Los Alamos National Lab. (United States)
Richard A. Keller, Los Alamos National Lab. (United States)
Alberto Haces, Life Technologies, Inc. (United States)
Po-Jen Shih, Life Technologies, Inc. (United States)
John D. Harding, Life Technologies, Inc. (United States)

Published in SPIE Proceedings Vol. 1891:
Advances in DNA Sequencing Technology
Richard A. Keller, Editor(s)

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