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Proceedings Paper

Genosensors: microfabricated devices for automated DNA sequence analysis
Author(s): Mitchell D. Eggers; Michael E. Hogan; Robert K. Reich; Jagannath B. Lamture; Ken Beattie; Mark A. Hollis; Daniel J. Ehrlich; Bernard B. Kosicki; John M. Shumaker; Raj S. Varma; Barry E. Burke; Al Murphy; Dennis D. Rathman
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Paper Abstract

A new technology is introduced for developing potentially low cost, high throughput DNA sequence analysis. This approach utilizes novel bioelectronic genosensor devices to rapidly detect hybridization events across a DNA probe array. Detection of DNA probe/target hybridization has been achieved by two electronic methods. The first method utilizes a permittivity chip which interrogates the miniature test fixtures with a low voltage alternating electric field. The second method, which is the emphasis of this paper, utilizes a charge- coupled device (CCD) to detect the hybridization of appropriately tagged (radioisotope, fluorescent, or chemiluminescent labels) target DNA to an array of DNA probes immobilized above the pixels. Such direct electronic-biologic coupling is shown to provide a tenfold sensitivity improvement over conventional lens-based detection systems.

Paper Details

Date Published: 24 June 1993
PDF: 14 pages
Proc. SPIE 1891, Advances in DNA Sequencing Technology, (24 June 1993); doi: 10.1117/12.146711
Show Author Affiliations
Mitchell D. Eggers, Houston Advanced Research Ctr. and Rice Univ. (United States)
Michael E. Hogan, Baylor College of Medicine (United States)
Robert K. Reich, Lincoln Lab./MIT (United States)
Jagannath B. Lamture, Baylor College of Medicine (United States)
Ken Beattie, Houston Advanced Research Ctr. and Baylor College of Medicine (United States)
Mark A. Hollis, Lincoln Lab./MIT (United States)
Daniel J. Ehrlich, Lincoln Lab./MIT (United States)
Bernard B. Kosicki, Lincoln Lab./MIT (United States)
John M. Shumaker, Houston Advanced Research Ctr. (United States)
Raj S. Varma, Baylor College of Medicine (United States)
Barry E. Burke, Lincoln Lab./MIT (United States)
Al Murphy, Lincoln Lab./MIT (United States)
Dennis D. Rathman, Lincoln Lab./MIT (United States)

Published in SPIE Proceedings Vol. 1891:
Advances in DNA Sequencing Technology
Richard A. Keller, Editor(s)

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