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Proceedings Paper

Time-of-flight mass spectrometry of DNA laser-ablated from frozen aqueous solutions: applications to the Human Genome Project
Author(s): Peter W. Williams; David Schieltz; Randall W. Nelson; Chau-Wen Chou; Cong-Wen Luo; Robert Thomas
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Paper Abstract

Techniques have been developed to volatilize intact massive DNA molecules using pulsed laser ablation of thin frozen films of aqueous DNA solutions. Electrophoresis assay of the ablated DNA shows that molecules as massive as approximately 400,000 Da can be ablated intact. It has been possible to obtain time-of-flight mass spectra of ablated multicomponent mixtures of single-stranded DNA with masses up to approximately 18,000 Da (a 60-nucleotide DNA oligomer). The possible application of time-of-flight mass spectrometry to the analysis and readout of DNA sequence mixtures, and the potential thereby to accelerate the Human Genome project, are discussed.

Paper Details

Date Published: 24 June 1993
PDF: 8 pages
Proc. SPIE 1891, Advances in DNA Sequencing Technology, (24 June 1993); doi: 10.1117/12.146708
Show Author Affiliations
Peter W. Williams, Arizona State Univ. (United States)
David Schieltz, Arizona State Univ. (United States)
Randall W. Nelson, Vestec Corp. (United States)
Chau-Wen Chou, Arizona State Univ. (United States)
Cong-Wen Luo, Arizona State Univ. (United States)
Robert Thomas, Arizona State Univ. (United States)

Published in SPIE Proceedings Vol. 1891:
Advances in DNA Sequencing Technology
Richard A. Keller, Editor(s)

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