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Proceedings Paper

Toward high-resolution imaging of DNA
Author(s): Fransiska S. Franke; David Keller
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Paper Abstract

Artificial SFM images of DNA calculated from molecular models show that very sharp tips are needed if the internal features of DNA molecules are to be resolved. The calculations are based on 'envelope image analysis', which takes into account only the geometric effect of a tip surface sliding over a sample surface. These calculations show that: (1) A tip of about 5 angstroms end radius of curvature is needed to profile the major groove of double stranded B- form DNA, and an even sharper tip is needed to profile the minor groove. A tip of 1.5 angstroms radius fully profiles the major and minor grooves, and distinguishes the phosphate groups along the backbone. A tip of 20 angstroms end radius shows only a smooth cylinder with no internal detail. (2) it is possible to distinguish various conformations of double- stranded DNA if the backbone spacing is resolved. This seems to require at least a 5 angstroms tip. (3) The individual bases of single-stranded DNA are not easily distinguishable even if the DNA is in a conformation where the bases lie flat on the substrate surface, and even if a very sharp 1.5 angstroms tip is used.

Paper Details

Date Published: 24 June 1993
PDF: 7 pages
Proc. SPIE 1891, Advances in DNA Sequencing Technology, (24 June 1993); doi: 10.1117/12.146707
Show Author Affiliations
Fransiska S. Franke, Univ. of New Mexico (United States)
David Keller, Univ. of New Mexico (United States)

Published in SPIE Proceedings Vol. 1891:
Advances in DNA Sequencing Technology
Richard A. Keller, Editor(s)

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