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Proceedings Paper

Potential applications of atomic force microscopy of DNA to the human genome project
Author(s): Helen G. Hansma; Paul K. Hansma
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Paper Abstract

A simple calculation shows that the information contained in the base sequence of the human genome could be recorded onto less than two compact discs. To read amounts of information comparable in size to the human genome, scanning probes are used routinely in both biology (i.e., living systems) and technology. The atomic force microscope (AFM) is a scanning probe that is now capable of imaging DNA routinely and reproducibly. The minimum size of structures seen reproducibly along DNA strands with the AFM is presently 2 to 3 nm, which is an order of magnitude less resolution than would be required to sequence DNA. At present, the AFM shows great potential for high-resolution mapping of DNA but is not capable of sequencing DNA without further improvements.

Paper Details

Date Published: 24 June 1993
PDF: 5 pages
Proc. SPIE 1891, Advances in DNA Sequencing Technology, (24 June 1993); doi: 10.1117/12.146705
Show Author Affiliations
Helen G. Hansma, Univ. of California/Santa Barbara (United States)
Paul K. Hansma, Univ. of California/Santa Barbara (United States)

Published in SPIE Proceedings Vol. 1891:
Advances in DNA Sequencing Technology
Richard A. Keller, Editor(s)

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