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Proceedings Paper

Intracellular events associated with uptake of a monomeric photosensitizer, benzoporphyrin derivative monoacid ring A (BPD), and its subsequent activation with light
Author(s): P. Mark Curry; Anna M. Richter; Ashok K. Jain; Julia G. Levy
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Paper Abstract

Intracellular events associated with photosensitizers and their subsequent activation with light are as yet poorly understood. Using a model photosensitizer, benzoporphyrin derivative, monoacid ring A (BPD), we have studied cellular uptake and release using both cell lines and normal murine splenocytes. These studies showed that maximum uptake was effected rapidly, peaking between 15 and 30 minutes following exposure to BPD. Absolute quantities taken up varies substantially between cell sources, malignant cell lines and activated normal cells taking up significantly higher levels. Release of BPD from cells occurred equally rapidly when they were removed from BPD-containing medium. Phototoxicity studies indicated that more sensitive cell structures were exposed to the photosensitizer with increased time of incubation. Further experiments were conducted to study protein expression in a murine tumor cell line following BPD treatment. We observed that the oxidative stress associated with photodynamic therapy resulted in the induction of a set of heat shock or stress proteins, and that the pattern of expression was similar when tumor cells were treated in vitro and in vivo.

Paper Details

Date Published: 18 June 1993
PDF: 13 pages
Proc. SPIE 1881, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy II, (18 June 1993); doi: 10.1117/12.146318
Show Author Affiliations
P. Mark Curry, Univ. of British Columbia (Canada)
Anna M. Richter, Univ. of British Columbia (Canada)
Ashok K. Jain, Univ. of British Columbia (Canada)
Julia G. Levy, Univ. of British Columbia (Canada)

Published in SPIE Proceedings Vol. 1881:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy II
Thomas J. Dougherty, Editor(s)

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