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Proceedings Paper

Imaging of superficially growing tumors by delayed observation of laser-induced fluorescence
Author(s): M. Kohl; Uwe Sukowski; Bernd Ebert; Joerg Neukammer; Herbert H. Rinneberg; Christian Nowak
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Paper Abstract

Images of superficially growing tumors marked with prophyrin-based photosensitizers (Photofrin II, P4P-mD) were recorded in vivo by delayed observation of fluorescence following pulsed laser excitation. A pulsed, solid state, widely tunable laser system was used together with an intensified CCD camera. The ratio of the fluorescence radiant exitances of tumor and normal tissue taken as contrast between tumor and surrounding tissue was studied systematically by varying excitation wavelength and delay time between the optical pulse and the opening of the intensifier of the CCD camera. The dependence of this ratio on excitation wavelength was explained qualitatively using a simple one-dimentional mathematical model, taking the penetration depth of the exciting laser radiation and the thickness of the tumor and the layer of normal tissue above it into account. Besides fluorescence images, delayed fluorescence emission spectra of tumor and normal tissue were recorded in vivo.

Paper Details

Date Published: 18 June 1993
PDF: 16 pages
Proc. SPIE 1881, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy II, (18 June 1993); doi: 10.1117/12.146312
Show Author Affiliations
M. Kohl, Physikalisch-Technische Bundesanstalt (Germany)
Uwe Sukowski, Physikalisch-Technische Bundesanstalt (Germany)
Bernd Ebert, Physikalisch-Technische Bundesanstalt (Germany)
Joerg Neukammer, Physikalisch-Technische Bundesanstalt (Germany)
Herbert H. Rinneberg, Physikalisch-Technische Bundesanstalt (Germany)
Christian Nowak, Max-Delbrueck-Centrum fuer Molekulare Medizin (Germany)

Published in SPIE Proceedings Vol. 1881:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy II
Thomas J. Dougherty, Editor(s)

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