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Proceedings Paper

Laser experiments on DNA electrophoretic mobilities
Author(s): Benjamin Chu; Evgeny V. Sokolov; A. R. Khokhlov
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Paper Abstract

By combining fluorescence recovery after photobleaching with gel electrophoresis, known as movements of fluorescence pattern after photobleaching (MOFPAP), it is possible to measure the time-dependent electrophoretic mobility of stained DNA fragments in a gel over relatively short time periods. The creation of a narrower stained DNA band by photobleaching can also shorten the running time and increase the resolution in a gel electrophoresis experiment for analytical purposes. In addition, a frequency analysis of MOFPAP signals has been simulated to demonstrate the potential capability of MOFPAP as an analytical tool for determining the presence of multiple species of DNA fragments in gel electrophoresis by measuring the electrophoretic mobility of each species present without spatial separation of different DNA fragments.

Paper Details

Date Published: 17 June 1993
PDF: 6 pages
Proc. SPIE 1922, Laser Study of Macroscopic Biosystems, (17 June 1993); doi: 10.1117/12.146218
Show Author Affiliations
Benjamin Chu, SUNY/Stony Brook (United States)
Evgeny V. Sokolov, SUNY/Stony Brook (United States)
A. R. Khokhlov, Moscow State Univ. (Russia)

Published in SPIE Proceedings Vol. 1922:
Laser Study of Macroscopic Biosystems
Jouko E. Korppi-Tommola, Editor(s)

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