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Proceedings Paper

Confocal fluorescence microscopy of living cells
Author(s): Silvia M. Doglia; L. Bianchi; Roberto Colombo; N. Allam; Hamid Morjani; Michel Manfait; A. M. Villa
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Paper Abstract

We present here the study of intracellular distribution of doxorubicin in the single living cell by scanning confocal fluorescence microscopy, with argon laser excitation and photon counting detection. New results on the nuclear and cytoplasmic drug fluorescence not detectable by conventional microscopy are discussed. Differences in the fluorescence pattern observed in living and fixed cells suggest new insights in the mode of action of the drug.

Paper Details

Date Published: 17 June 1993
PDF: 9 pages
Proc. SPIE 1922, Laser Study of Macroscopic Biosystems, (17 June 1993); doi: 10.1117/12.146189
Show Author Affiliations
Silvia M. Doglia, Univ. of Milan (Italy)
L. Bianchi, Consorzio Bioricerche (Italy)
Roberto Colombo, Univ. of Milan (Italy)
N. Allam, Univ. of Reims (France)
Hamid Morjani, Univ. of Reims (France)
Michel Manfait, Univ. of Reims (France)
A. M. Villa, Consorzio Bioricerche (Italy)

Published in SPIE Proceedings Vol. 1922:
Laser Study of Macroscopic Biosystems
Jouko E. Korppi-Tommola, Editor(s)

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