A quantitative study of the rise, magnitude, and decay of birefringence of DNA in agarose gel under an electric pulse E has been performed. It provides us with the molecular weight dependence of the characteristic times which describe the deformation of the chain during the transport and which are the parameters requested for designing electric pulse programs for pulsed field gel electrophoresis capable of achieving separation of DNA fragments of very high molecular weight.

Date Published: 17 June 1993
PDF: 9 pages
Proc. SPIE 1922, Laser Study of Macroscopic Biosystems, (17 June 1993); doi: 10.1117/12.146161

Published in SPIE Proceedings Vol. 1922:
Laser Study of Macroscopic Biosystems
Jouko E. Korppi-Tommola, Editor(s)