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Proceedings Paper

Characterization of photosynthetic reaction centers by surface-enhanced resonance Raman scattering
Author(s): George D. Chumanov; Therese M. Cotton; Chengli Zhou; Dale Gaul; Rafael Picorel; Michael Seibert
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Paper Abstract

Surface-enhanced Resonance Raman scattering (SERRS) spectra were obtained for the reaction center complexes of the photosynthetic bacterium Rhodobacter sphaeroides (RC) and from photosystem II (PSII) of spinach, adsorbed on Ag and Au surfaces. These preliminary results demonstrate the considerable potential of this technique for selectively exciting resonance Raman scattering from reaction center components within their distinct absorption bands. Because of the high sensitivity afforded by SERRS, spectra could be measured from a single monolayer of reaction centers adsorbed on a metal surface. The surface-sensitivity provides new information indicating the topology of the PSII reaction center 47 kD light-harvesting protein complex. The activity of the PSII reaction center complex adsorbed on metal surfaces was monitored by photochemical reduction of cyt b-559. Measurement of fluorescence emission was shown to be a new and sensitive method for monitoring the structural and functional integrity of the PSII reaction center complex on the metal surface.

Paper Details

Date Published: 17 June 1993
PDF: 9 pages
Proc. SPIE 1921, Laser Spectroscopy of Biomolecules, (17 June 1993); doi: 10.1117/12.146131
Show Author Affiliations
George D. Chumanov, Iowa State Univ. (United States)
Therese M. Cotton, Iowa State Univ. (United States)
Chengli Zhou, Iowa State Univ. (United States)
Dale Gaul, Iowa State Univ. (United States)
Rafael Picorel, CSIC (Spain)
Michael Seibert, National Renewable Energy Lab. (United States)

Published in SPIE Proceedings Vol. 1921:
Laser Spectroscopy of Biomolecules
Jouko E. Korppi-Tommola, Editor(s)

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