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Proceedings Paper

Analysis of excitation and collection geometries for planar waveguide immunosensors
Author(s): Douglas A. Christensen; Shellee Dyer; David Fowers; James N. Herron
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Paper Abstract

We demonstrate the use of a two-channel flowcell for fluorescent immunoassays. The flowcell contains a planar silica waveguide for evanescent excitation of the fluorophores, and the planar waveguide surface provides the solid support for immobilization of the antibodies. The detection system is composed of a grating spectrometer and a CCD camera for spectral characterization of the emitted signals. Two methods of sensing have been studied: a displacement-type technique and a sandwich-type assay. The sensitivity achieved for measuring concentrations of HCG by the sandwich method is sub-picomolar. Also, we have experimentally compared the signal strengths for two alternative ways of excitation and collection, and determine that waveguide excitation/side collection has some practical advantages over side excitation/waveguide collection.

Paper Details

Date Published: 21 May 1993
PDF: 7 pages
Proc. SPIE 1886, Fiber Optic Sensors in Medical Diagnostics, (21 May 1993); doi: 10.1117/12.144833
Show Author Affiliations
Douglas A. Christensen, Univ. of Utah (United States)
Shellee Dyer, Univ. of Utah (United States)
David Fowers, Univ. of Utah (United States)
James N. Herron, Univ. of Utah (United States)

Published in SPIE Proceedings Vol. 1886:
Fiber Optic Sensors in Medical Diagnostics
Fred P. Milanovich, Editor(s)

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