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Proceedings Paper

Time-correlated photon counting: differential methods applied to anisotropy decay
Author(s): Douglas Magde; Michael D. Magde
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Paper Abstract

A method is described that makes it possible to measure luminescence decays for both parallel and perpendicular excitation geometries in a single experimental run. The rotational diffusion of oxazine 725 in five solvents of different viscosities is characterized using the new procedure. The measurements are challenging in that the rotational diffusion times are close to the excited state lifetime. The advantage of the new procedure lies in the increased confidence that comes with measuring both polarizations under the same conditions. The same principle is applicable in any circumstance involving the comparison of two or more samples.

Paper Details

Date Published: 18 May 1993
PDF: 10 pages
Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); doi: 10.1117/12.144727
Show Author Affiliations
Douglas Magde, Univ. of California/San Diego (United States)
Michael D. Magde, Univ. of California/San Diego (United States)

Published in SPIE Proceedings Vol. 1885:
Advances in Fluorescence Sensing Technology
Joseph R. Lakowicz; Richard B. Thompson, Editor(s)

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