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Proceedings Paper

Temporal and spectral separation of singlet oxygen luminescence from near-infrared emitting photosensitizers
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Paper Abstract

The luminescence emission of singlet molecular oxygen (1O2) generated by bacteriopheophytin a, a near infrared emitting photosensitizer, was measured using a new high sensitivity spectrometer system for time and spectral resolved near infrared detection. The instrument uses a low energy pulsed nitrogen laser (40 (mu) J per pulse) to optically excite the photosensitizer and is capable of a time resolution of 40 ns per data point and an instrument response function of 350 ns FWHM (full width at half maximum). The use of a low energy (and relatively low cost) source provides sufficient system sensitivity to measure time resolved spectra in the near infrared with high spectral and temporal resolution. The simultaneous detection, with high accuracy and repeatability, of both the temporal and spectral dependence of the photo-processes of 1O2 generation, especially with near infrared emitting photosensitizers, may further stimulate the current intensive investigations concerning the activity of 1O2 to bio-molecules.

Paper Details

Date Published: 18 May 1993
PDF: 12 pages
Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); doi: 10.1117/12.144726
Show Author Affiliations
John R. Gilchrist, Edinburgh Instruments Ltd. (United States)
Dirk U. Nather, Edinburgh Instruments Ltd. (United Kingdom)

Published in SPIE Proceedings Vol. 1885:
Advances in Fluorescence Sensing Technology
Joseph R. Lakowicz; Richard B. Thompson, Editor(s)

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