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Proceedings Paper

Time-resolved immunofluorimetic assay (TRIFMA): diagnostic method of the future
Author(s): U. Missler; U. Gaida; Hong Li; W. G. Wood
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Paper Abstract

This article describes the development and clinical evaluation of two-site immunometric assays for ferritin, thyrotropin (TSH), and tumor necrosis factor alpha (TNF(alpha) ) using time resolved fluorescent measurement with streptavidin-europium (STAV-Eu+3) as a label. The liquid phase antibodies were labeled with amidocaproylbiotin-N- hydroxysuccinimide ester. All three assays were based on microtiterplate technology and could be completed within a working day (incubation times less than 4 h). The ferritin assay was compared with luminescent and enzyme labeled assays using identical components. The TSH assay was compared with a commercial immunoluminometric assay whereas the TNF assay was unable to be compared with another method, only with standards from an independent source. The performance data was excellent with lower detection limits for TSH from < 0.003 mU/l and for TNF(alpha) under 10 ng/l. Intra-assay precision was acceptable within the range of interest with TSH < 4% (0.2 - 50 mU/l), TNF(alpha) < 15% (70 - 8000 ng/l and ferritin < 8% (10 - 500 g/l). Inter-assay precision was < 6% for TSH, < 16% for TNF(alpha) and < 8.5% for ferritin. All assays were performed using commercially available components and proved suitable for routine use.

Paper Details

Date Published: 18 May 1993
PDF: 13 pages
Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); doi: 10.1117/12.144710
Show Author Affiliations
U. Missler, Medical Univ. of Luebeck (Germany)
U. Gaida, Medical Univ. of Luebeck (Germany)
Hong Li, Zhejiang Medical Univ. (China)
W. G. Wood, Stralsund City Hospital (Germany)

Published in SPIE Proceedings Vol. 1885:
Advances in Fluorescence Sensing Technology
Joseph R. Lakowicz; Richard B. Thompson, Editor(s)

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