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Proceedings Paper

Development of a flow injection immunoanalysis (FIIA) for pesticide determination by means of fluorescence detection
Author(s): Monika Wortberg; Cornelia Middendorf; Joerg Krause; Karl Cammann
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Paper Abstract

In this paper we describe the state of development of a flow-through immunosensor for quasi- continuous monitoring of pesticides, especially triazine herbicides, in drinking water. Starting with time-resolved fluoroimmunoassays using Eu-chelates as fluorescent labels, we obtained a detection limit of 0.1 (mu) g/l for some triazine herbicides. The experimental parameters applied in assays on microtiter strips were transferred and adjusted to small carrier beads as solid phase in affinity columns. For fluorescence detection in the FIIA a pulsed laser was used as an excitation source. The light was coupled into a flow-through cuvette and the fluorescence signal was recorded with a photomultiplier tube working in a gated detection mode.

Paper Details

Date Published: 18 May 1993
PDF: 3 pages
Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); doi: 10.1117/12.144708
Show Author Affiliations
Monika Wortberg, Univ. Muenster (United States)
Cornelia Middendorf, Institut fuer Chemo- and Biosensorik (Germany)
Joerg Krause, Univ. Muenster (Germany)
Karl Cammann, Univ. Muenster and Institut fuer Chemo- and Biosensorik (Germany)

Published in SPIE Proceedings Vol. 1885:
Advances in Fluorescence Sensing Technology
Joseph R. Lakowicz; Richard B. Thompson, Editor(s)

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