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Proceedings Paper

Optical triple sensor for measuring pH, oxygen, and carbon dioxide in bioreactors
Author(s): Bernhard H. Weigl; Andrej Holobar; Wolfgang Trettnak; Ingo Klimant; H. Kraus; Paul O'Leary; Otto S. Wolfbeis
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Paper Abstract

A triple sensor unit consisting of opto-chemical sensors for measurement of pH, oxygen, and carbon dioxide is presented. The pH sensor and the CO2 sensor are based on the color change of a pH-sensitive dye immobilized on a polymeric support. The resulting changes in absorption are monitored through optical fibers at one or two analytical wavelengths. The oxygen sensor is based on the quenching of the fluorescence of a metalorganic dye. The operation principle and the performance of all three sensors are described thoroughly with respect to their application in bioreactors. All three sensors are fully LED compatible. The chemical and mechanical stability, especially against common sterilization methods, are described in some detail. A calibration and measurement software comprising fit routines for the sensors and a mathematical treatment of the results are presented as well.

Paper Details

Date Published: 30 April 1993
PDF: 11 pages
Proc. SPIE 1796, Chemical, Biochemical, and Environmental Fiber Sensors IV, (30 April 1993); doi: 10.1117/12.143538
Show Author Affiliations
Bernhard H. Weigl, Joanneum Research and Karl-Franzens-Univ. Graz (Australia)
Andrej Holobar, Joanneum Research and Karl-Franzens-Univ. Graz. (Australia)
Wolfgang Trettnak, Karl-Franzens-Univ. Graz (Austria)
Ingo Klimant, Karl-Franzens-Univ. Graz (Austria)
H. Kraus, Joanneum Research (Austria)
Paul O'Leary, Karl-Franzens-Univ. Graz (Austria)
Otto S. Wolfbeis, Karl-Franzens Univ. Graz (Austria)

Published in SPIE Proceedings Vol. 1796:
Chemical, Biochemical, and Environmental Fiber Sensors IV
Robert A. Lieberman, Editor(s)

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