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Proceedings Paper

Development of an enzyme immunoassay for the determination of 2,4,6-trinitrotoluene: probing the influences of humic acids
Author(s): Claudia Keuchel; Ludwig Weil; Reinhard Niessner
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Paper Abstract

A new enzyme immunoassay is described which can be used for the determination of the explosive 2,4,6-trinitrotoluene in water. The assay has a detection limit of 30 ng/l. The evaluation of cross-reactivities shows that the used antibody has a high selectivity for the trinitrophenyl moiety. Since humic substances, commonly occurring in natural waters, can interact with antibodies, the obtained results can be false positive. To reduce the negative influence of the humic substances the usually performed ELISA protocol has been modified by adding a solution of bovine serum albumin (0.5% in PBS) after the coating step.

Paper Details

Date Published: 9 March 1993
PDF: 7 pages
Proc. SPIE 1716, International Conference on Monitoring of Toxic Chemicals and Biomarkers, (9 March 1993); doi: 10.1117/12.140237
Show Author Affiliations
Claudia Keuchel, Technische Univ. Muenchen (Germany)
Ludwig Weil, Technische Univ. Muenchen (Germany)
Reinhard Niessner, Technische Univ. Muenchen (Germany)

Published in SPIE Proceedings Vol. 1716:
International Conference on Monitoring of Toxic Chemicals and Biomarkers
Tuan Vo-Dinh; Karl Cammann, Editor(s)

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