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Proceedings Paper

Role of x-ray-induced damage in biological micro-imaging
Author(s): Richard A. London; James E. Trebes; Chris J. Jacobsen
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Paper Abstract

Interactions of x rays with a sample are studied to determine the optimal wavelength, source energy, and exposure time for microcopy and holography. The optimal wavelength is influenced by two criteria: minimizing the required source energy and minimizing the absorbed dose and subsequent damage to the sample. The use of heavy element labels, such as colloidal gold, can significantly reduce the energy and dose. Limits to the exposure time due to natural motions, x-ray induced chemical damage, heat build-up, and hydrodynamic expansion are discussed.

Paper Details

Date Published: 13 January 1993
PDF: 8 pages
Proc. SPIE 1741, Soft X-Ray Microscopy, (13 January 1993); doi: 10.1117/12.138749
Show Author Affiliations
Richard A. London, Lawrence Livermore National Lab. (United States)
James E. Trebes, Lawrence Livermore National Lab. (United States)
Chris J. Jacobsen, SUNY/Stony Brook (United States)

Published in SPIE Proceedings Vol. 1741:
Soft X-Ray Microscopy
Chris J. Jacobsen; James E. Trebes, Editor(s)

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