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Proceedings Paper

Experiments in scanning luminescence x-ray microscopy
Author(s): Chris J. Jacobsen; Steve A. Lindaas; Vivian Oehler; Shawn P. Williams; Sue Wirick; Xiaodong Zhang; Shirong Guo; Ilan Spector
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Paper Abstract

Scanning luminescence x-ray microscopy is based on collecting visible light emission from a sample region illuminated by an x-ray microprobe. We have tested the resolution of the method using P31 phosphor grains, and have obtained luminescence images of dye-labelled polystyrene spheres and sodium salicylate crystals. However, we have observed no light emission from the dyes DAPI, ethidium bromide, Hoecst 33258, and rhodamine phalloidon. Present efforts are aimed at improving our understanding of the luminescence process so as to find appropriate dyes for imaging dye-labelled biological specimens.

Paper Details

Date Published: 13 January 1993
PDF: 9 pages
Proc. SPIE 1741, Soft X-Ray Microscopy, (13 January 1993); doi: 10.1117/12.138735
Show Author Affiliations
Chris J. Jacobsen, SUNY/Stony Brook (United States)
Steve A. Lindaas, SUNY/Stony Brook (United States)
Vivian Oehler, SUNY/Stony Brook (United States)
Shawn P. Williams, SUNY/Stony Brook (United States)
Sue Wirick, SUNY/Stony Brook (United States)
Xiaodong Zhang, SUNY/Stony Brook (United States)
Shirong Guo, SUNY/Stony Brook (United States)
Ilan Spector, SUNY/Stony Brook (United States)

Published in SPIE Proceedings Vol. 1741:
Soft X-Ray Microscopy
Chris J. Jacobsen; James E. Trebes, Editor(s)

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